To make this Cowboy Cornbread Trifle gluten-free, substitute the Jiffy cornbread mix with a certified gluten-free cornbread mix. Confirm that the chili beans, ranch dressing, mayonnaise, and all processed ingredients (especially bacon and shredded cheese) are labeled gluten-free, as some may include additives or be processed in facilities that also handle wheat. Use gluten-free labels to ensure full compliance.